Immobilized Metal Ion Chromatography

1974 First use of immobilized chelators to isolate metalloproteins 1975 First description of general technique (IMAC) using IDA 1983 Introduction of high performance on silica based media 1986 Use of Fe3# chelates to purify phosphoproteins 1987 Introduction of NTA 1988 Introduction of genetically engineered His tags 1992 Introduction of TREN 1998 Introduction of TACN Freeze NH (1995) Lectin afRnity chromatography. Protocols in Protein Chemistry, pp. 901}919. NewYork:Wiley. Gerard C (1990) PuriRcation of glycoproteins. In: Deutscher MP (ed.) Guide to Protein PuriTcation, Methods in Enzymology, vol. 182. New York: Academic Press. Liu X-C and ScoutenWH (1994) New ligands for boronate afRnity chromatography. Journal of Chromatography A, 687: 61}69. Liu X-C and Scouten WH (1996) Studies on oriented and reversible immobilization of glycoprotein using novel boronate afRnity gel. Journal of Molecular Recognition, 9: 462}467. West I and Goldring O (1996) Lectin afRnity chromatography. In: Doonan S (ed.) Methods in Molecular Biology, Protein PuriTcation Protocols, vol. 59, pp. 177}185. New Jersey: Humana Press.